LBH

Ho kopanya li-centrifuges ho phallo ea mosebetsi ea laboratori ho hloka moralo o hlokolosi le khokahanyo ho netefatsa ts'ebetso e nepahetseng le tlhahiso.

Malebela ke ana a akaretsang a ho kopanya li-centrifuges ho phallo ea mosebetsi ea laboratori:

1. Hlalosa litlhoko tsa centrifugation: Hlalosa mefuta ea lisampole le litlhoko tsa centrifugation bakeng sa phallo ea mosebetsi ea laboratori, joalo ka lebelo le hlokahalang, nako, le matla a centrifugation. Sena se tla u thusa ho khetha mohlala o nepahetseng oa centrifuge le lisebelisoa.

2. Hlahisa mekhoa e tloaelehileng ea ts'ebetso: Ntlafatsa mekhoa e tloaelehileng ea ts'ebetso (SOPs) bakeng sa li-protocol tsa centrifugation, ho kenyelletsa le ho lokisetsa sampuli, ho kenya le ho theola, hammoho le mekhoa ea ho hloekisa le ho e lokisa. Koetlisa basebetsi bohle ba lab ho li-SOP tsena ho netefatsa liphetho tse tsitsitseng le tse nepahetseng.

3. Ntlafatsa tlhahiso ea sampole: Ntlafatsa tlhahiso ea sampole ka ho kopanya lisampole tse tšoanang hammoho le ho rera kemiso ea centrifugation ka nepo. Sena se ka thusa ho fokotsa nako le lisebelisoa tse hlokahalang bakeng sa centrifugation le ho ntlafatsa katleho ea lab ka kakaretso.

4. Hokahanya le mesebetsi e meng ea lab: Ho hokahanya le mesebetsi e meng ea lab, joalo ka pokello ea sampole, ts'ebetso le tlhahlobo, ho etsa bonnete ba hore phallo ea mosebetsi oa centrifugation e hokahane hantle le phallo ea mosebetsi ea lab ka kakaretso. Nahana ka ho sebelisa lisebelisoa tse ikemetseng kapa tsa liroboto ho ntlafatsa ts'ebetso.

5. Netefatsa tlhokomelo e nepahetseng le ho lekanyetsoa: Netefatsa tlhokomelo e nepahetseng le tekano ea li-centrifuge tsa hau ho netefatsa liphetho tse nepahetseng le tse tšepahalang. Etsa moralo oa kamehla oa ho hlahloba le ho hlahloba boemo, 'me u etse mekhoa ea ho hloekisa le ho bolaea likokoana-hloko ho thibela tšoaetso le ho netefatsa ts'ebetso e nepahetseng.

6. Lekola le ho lekola tšebetso: Lekola le ho lekola tšebetso ea li-centrifuge tsa hau khafetsa, joalo ka ho sala morao palo ea li-run, lirekoto tsa tlhokomelo le tokiso, le maikutlo a basebelisi. Sebelisa lintlha tsena ho tseba mathata kapa libaka tseo u ka li ntlafatsang, 'me u lokise mokhoa oa hau oa ho sebetsa ka nepo.

Hopola hore litlhoko tse ikhethileng le phallo ea mosebetsi bakeng sa ho kopanya li-centrifuges ka laboratoring ea hau li ka fapana ho latela mofuta le boholo ba lipatlisiso tsa hau. Sheba litataiso le mekhoa e metle, 'me u batle maikutlo ho tsoa ho basebetsi le litsebi tse ling ho ntlafatsa ts'ebetso ea hau ea centrifugation.

Ho boloka le ho tsamaisa lisampole ka mor'a centrifugation ho hloka ho tšoaroa ka hloko ho qoba ho senyeha kapa ho silafala ha sampole.

Malebela ke ana a akaretsang a ho boloka le ho tsamaisa lisampole tsa hau tsa centrifuged:

1. Ngola disampole tsa hao: Ngola disampole tsa hao tse centrifuged ka ho hlaka ho latela letsatsi, nako, le tlhahisoleseding e nngwe e amehang, jwalo ka mofuta wa sampole le maemo a centrifugation.

2. Sebelisa lijana tse loketseng tsa polokelo: Khetha lijana tse loketseng tsa polokelo ho latela mofuta le bophahamo ba sampole ea hau. Ho etsa mohlala, sebelisa li-tube kapa li-vial tse hloekisitsoeng bakeng sa lisampole tsa baeloji le lijana tse sa keneng moea bakeng sa lisampole tse sa tsitsang kapa tse bobebe.

3. Boloka disampole mochesong o loketseng: Boloka disampole tsa hao mochesong o loketseng ho thibela ho senyeha kapa ho senyeha ha disampole. Bakeng sa mehlala ea likokoana-hloko, li boloke mocheso o khothalletsoang, o kang -80°C bakeng sa polokelo ea nako e telele kapa -20°C bakeng sa polokelo ea nakoana. Bakeng sa mefuta e meng ea lisampole, sheba litataiso tse amehang kapa lingoliloeng bakeng sa maemo a khothaletsoang a polokelo.

4. Sireletsa disampole leseding: Haeba disampole tsa hao di sa utlwelle kganya, di boloke ka dijaneng tse opaque kapa o di phuthele ka foile ya aluminium ho di sireletsa kganyeng ya kganya.

5. Lisampole tsa lipalangoang holim'a leqhoa: Haeba u hloka ho tsamaisa lisampole tsa hau, sebelisa leqhoa kapa leqhoa le omileng ho boloka mocheso o loketseng. Etsa bonnete ba hore lisampole li ngotsoe ka nepo 'me li pakiloeng ka mokhoa o sireletsehileng ho thibela ho qhalana kapa ho lutla.

6. Fokotsa linako tsa ho qhibiliha ha leqhoa: Haeba u hloka ho qhibiliha lisampole bakeng sa tlhahlobo e eketsehileng kapa liteko, fokotsa palo ea li-cycles tsa leqhoa ho qoba ho senyeha ha mehlala. Boloka lisampole ka li-aliquot tse nyane ho fokotsa palo ea sampole e hlokang ho qhibiliha hang-hang.

7. Bea leihlo boleng ba sampole: Nako le nako hlokomela boleng le botšepehi ba lisampole tsa hau tse bolokiloeng ka ho etsa liteko tsa taolo ea boleng, joalo ka spectrophotometry kapa mekhoa e meng ea tlhahlobo. Sena se ka u thusa ho lemoha liphetoho leha e le life kapa ho senyeha ho lisampole tsa hau ha nako e ntse e ea.

Hopola hore maemo a ikhethileng a polokelo le lipalangoang a ka fapana ho latela mofuta le mofuta oa lisampole tsa hau. Kamehla sheba litataiso tse amehang kapa lingoliloeng 'me u latele mekhoa e metle ea ho boloka le ho tšoara sampole ho netefatsa bonnete le ts'epahalo ea liphetho tsa hau.

Ho hloekisa le ho bolaea centrifuge ea hau ho bohlokoa ho thibela tšilafalo le ho netefatsa bonnete ba liteko tsa hau.

Mehato e akaretsang ea ho hloekisa le ho bolaea centrifuge ea hau ke ena:

1. Koala le ho ntša centrifuge: Pele u hloekisa centrifuge ea hau, etsa bonnete ba hore ua e tima le ho e ntša polakeng ho qoba likotsi leha e le life tsa motlakase.

2. Tlosa lisampole leha e le life kapa litšila: Ka hloko tlosa lisampole leha e le life kapa lithōle ho centrifuge. Haeba ho na le ho qhalana kapa ho lutla hofe kapa hofe, li hloekise ka tharollo e loketseng ea ho hloekisa.

3. Hlakola centrifuge: Ho itšetlehile ka mofuta oa centrifuge, ho ka 'na ha hlokahala hore u e harole ho fihlella likarolo tsohle tse hlokang ho hloekisoa. Sheba litaelo tsa moetsi kapa bukana ea mosebelisi bakeng sa tataiso mabapi le mokhoa oa ho harola centrifuge.

4. Hloekisa likarolo tsa centrifuge: Sebelisa motsoako o bonolo oa sesepa le borashe bo bobebe kapa lesela ho hloekisa likarolo tsa centrifuge. Ela hloko ka ho khethehileng libaka tseo ho leng thata ho fihla ho tsona kapa tseo e ka ’nang eaba li bokelletse lithōle kapa tse hahelletsoeng. Qoba ho sebelisa lihloekisi tse hlabang kapa lisebelisoa tse ka ngopang kapa tsa senya bokaholimo ba centrifuge.

5. Hlatsoa le ho omisa likarolo tsa centrifuge: Ka mor'a ho hloekisa, hlatsoa likarolo tsa centrifuge ka metsi a hloekileng 'me u li omise hantle ka lesela le hloekileng, le se nang lesela. Etsa bonnete ba hore ha ho na mongobo o setseng o setseng likarolong.

6. Hlakola likarolo tsa centrifuge: Sebelisa tharollo e loketseng ea sebolaea-mafu ho bolaea likarolo tsa centrifuge. Latela litaelo tsa moetsi bakeng sa ho hlapolla, nako ea ho ikopanya le mokhoa oa ts'ebeliso. Netefatsa hore tharollo ea sebolaea-mafu e fihla likarolong tsohle tsa centrifuge tseo e kanna eaba li kopane le lisampole tsa hau.

7. Hlatsoa le ho omisa likarolo tsa centrifuge hape: Ka mor'a ho bolaea likokoana-hloko, hlatsoa likarolo tsa centrifuge ka metsi a hloekileng 'me u li omise ka botlalo ka lesela le hloekileng, le se nang lesela. Hape, etsa bonnete ba hore ha ho na mongobo o setseng o setseng likarolong.

8. Kopanya centrifuge hape: Hang ha likarolo tsa centrifuge li omme, kopanya hape centrifuge ho ea ka litaelo tsa moetsi. Etsa bonnete ba hore likarolo tsohle li sirelelitsoe hantle 'me li tiisitsoe.

9. Netefatsa ts'ebetso ea centrifuge: Pele o sebelisa centrifuge hape, netefatsa ts'ebetso ea eona ka ho sebelisa tlhahlobo ea centrifuge ka mohlala oa taolo.

Hlokomela hore mekhoa e ikhethileng ea ho hloekisa le ho bolaea likokoana-hloko e ka fapana ho latela mofuta le mohlala oa centrifuge ea hau, hammoho le mofuta oa lisampole tsa hau. Kamehla sheba litaelo tsa moetsi kapa bukana ea mosebelisi bakeng sa tataiso mabapi le mokhoa oa ho hloekisa le ho bolaea likokoana-hloko mohlala oa hau oa centrifuge.

Ho toloka le ho sekaseka liphetho tsa centrifuge ho kenyelletsa ho utloisisa likarolo tse fapaneng tsa sampole ea hau le kabo ea tsona kamora centrifugation.

Mona ke tataiso e akaretsang mabapi le mokhoa oa ho toloka le ho sekaseka liphetho tsa centrifuge:

1. Hlahloba likarolo tse arohaneng: Ka mor'a centrifugation, tlosa ka hloko li-tubes kapa lijana ho tloha centrifuge 'me u hlahlobe likarolo tse arohaneng. Hlokomela 'mala oa tsona, sebōpeho le bophahamo ba tsona.

2. Lekanya mahloriso a likaroloana: Sebelisa spectrophotometer kapa lisebelisoa tse ling tsa ho hlahloba ho lekanya khatello ea likarolo tse arohaneng. Sena se ka u fa maikutlo a bohloeki le chai ea karolo ka 'ngoe.

3. Sekaseka kabo ea likaroloana: Haeba u arotse likarolo tse ngata sampoleng ea hau, sekaseka kabo ea karolo ka 'ngoe makaleng a fapaneng a tšupu ea centrifuge kapa sets'oants'o. Sena se ka u thusa ho tseba bongata bo lekanyelitsoeng ba karolo ka 'ngoe ea sampole ea hau.

4. Bapisa liphetho tsa hau le khopolo-taba ea hau: Haeba u ne u e-na le khopolo e itseng bakeng sa teko ea hau, bapisa liphetho tsa hau le khopolo ea hau. Na u fihletse karohano e lebelletsoeng ea likarolo? Na ho bile le liphumano tse neng li sa lebelloa kapa liphoso liphethong tsa hau?

5. Pheta teko: Ho netefatsa ho tšepahala ha liphetho tsa hau, pheta teko bonyane makhetlo a mararo 'me u bale palohare ea liphetho. Sena se ka u thusa ho fokotsa litlamorao tsa liphoso tsa liteko le ho eketsa bohlokoa ba lipalo tsa liphetho tsa hau.

6. Sebelisa tlhahlobo ea lipalo-palo: Sebelisa mekhoa e nepahetseng ea tlhahlobo ea lipalo-palo ho sekaseka liphetho tsa hau, joalo ka tlhahlobo ea t-test, ANOVA, kapa regression analysis. Mekhoa ena e ka u thusa ho tseba bohlokoa ba liphetho tsa hau le ho tseba likamano leha e le life lipakeng tsa mefuta e fapaneng.

7. Hlalosa liphetho: Qetellong, toloka liphetho ho latela maikutlo a hau le potso ea lipatlisiso. Liphetho li fana ka maikutlo afe mabapi le thepa kapa litšobotsi tsa sampole ea hau? Litlamorao tsa liphetho tsa hau bakeng sa lipatlisiso kapa likopo tsa nako e tlang ke life?

Hopola hore ho toloka le ho sekaseka liphetho tsa centrifuge ho ka ba thata mme ho ka hloka tsebo e khethehileng le boiphihlelo. Haeba o sa tiisehe mabapi le mokhoa oa ho sekaseka liphetho tsa hau, batla keletso ho setsebi se hloahloa kapa sheba lingoliloeng tsa saense tse amehang.

Ho bala matla a hlokahalang a g-force bakeng sa teko ea centrifuge ho bohlokoa ho netefatsa hore centrifuge e khona ho finyella karohano e hlokahalang ea likarolo tsa sampole ea hau.

Mona ke mokhoa oa ho bala g-force:

1. Fumana matla a karohano a hlokehang: Matla a karohano a hlokehang bakeng sa teko ea hau a itšetlehile ka thepa ea sampuli ea hau. Ka mohlala, haeba u batla ho arola lisele tsa mali ho plasma, u tla hloka matla a karohano a phahameng ho feta ha u ne u arola lisele tsa baktheria ho setso sa metsi.

2. Fetolela matla a karohano a hlokehang ho g-force: Matla a karohano hangata a lekanngoa ka li-unit tsa Newtons (N) kapa pounds-force (lbf). Ho fetolela sena ho g-force, o hloka ho arola matla ka boima ba sampole. Boima ba sampuli bo lekana le boima ba sampuli bo atisitsoeng ke ho potlakisa ka lebaka la matla a khoheli (9.81 m/s²).

Mohlala, haeba o hloka matla a karohano a 100 N bakeng sa sampole ea hau, 'me sampole e na le boima ba 1 kg, matla a g a hlokahalang e tla ba:

G-force = Matla a karohano / (Boima ba mohlala)

G-force = 100 N / (1 kg x 9.81 m/s²)

G-matla = 10.17 g

Ka hona, o tla hloka centrifuge e ka hlahisang bonyane 10.17 g ea matla ho fihlela karohano e hlokahalang tekong ea hau.

Hlokomela hore li-centrifuge tse fapaneng li na le lebelo le phahameng le fapaneng la matla a g, kahoo etsa bonnete ba hore u khetha centrifuge e khonang ho fumana matla a g-force a hlokahalang bakeng sa teko ea hau. Hape, ela hloko hore ho feta matla a g-force a khothaletsoang bakeng sa lisampole tsa hau ho ka baka tšenyo kapa hona ho li senya, kahoo etsa bonnete ba hore ha u fete meeli e khothaletsoang.

Li-Centrifuge ke lisebelisoa tsa bohlokoa tsa laboratori tse sebelisetsoang ho arola likarolo tse fapaneng tsa lisampole. Joalo ka lisebelisoa tse ling, li-centrifuges li ka ba le mathata a ka amang ts'ebetso ea tsona.

Mona ke mathata a mang a tloaelehileng ka li-centrifuge le mokhoa oa ho li rarolla le ho li lokisa:

1. Setsi sa centrifuge se ke ke sa qala kapa sa bilika:

* Lekola hore na thapo ea motlakase e hoketsoe hantle le sesebelisoa sa motlakase se butsitsoe.

* Netefatsa hore sekwahelo se koetsoe ka mokhoa o sireletsehileng 'me senotlolo sa polokeho se sebetsa ka nepo.

* Sheba hore na rotor e kentsoe hantle ebile e leka-lekane.

* Lekola fuse kapa sekhechana sa potoloho ebe u se fetola ha ho hlokahala.

* Haeba ho se le e 'ngoe ea litokiso tsena e sebetsang, e kanna ea ba bothata ka enjene, 'me u lokela ho ikopanya le setsebi.

2. Setsi se etsa lerata le sa tloaelehang:

* Lekola rotor bakeng sa matšoao leha e le afe a bonahalang a tšenyo kapa ho loana.

* Etsa bonnete ba hore rotor e leka-lekane hantle ebile e kentsoe.

* Sheba li-bearings bakeng sa matšoao a ho tsofala kapa a senyehileng, 'me u li fetole ha ho hlokahala.

* Sheba hore na centrifuge e boemong bo botle, 'me u fetole haeba ho hlokahala.

3. Setsi sa centrifuge se thothomela ho feta tekano:

* Sheba hore na centrifuge e boemong bo botle, 'me u fetole haeba ho hlokahala.

* Etsa bonnete ba hore rotor e leka-lekane hantle ebile e kentsoe.

* Sheba li-bearings bakeng sa matšoao a ho tsofala kapa a senyehileng, 'me u li fetole ha ho hlokahala.

4. Setsi sa centrifuge ha se fihlelle lebelo kapa matla a lakatsehang:

* Sheba hore na rotor e lumellana le centrifuge.

* Sheba hore na rotor e leka-lekana hantle ebile e kentsoe.

* Sheba hore na centrifuge e boemong bo botle, 'me u fetole haeba ho hlokahala.

* Lekola hore na brake e sebetsa hantle le hore ha e sebetse pele ho nako.

5. Setsi sa centrifuge ha se pholise:

* Lekola hore na sistimi ea sehatsetsi e buletsoe le ho sebetsa ka nepo.

* Lekola boemo ba sehatsetsi 'me u tlatse hape ha ho hlokahala.

* Lekola condenser bakeng sa matšoao a tšitiso kapa tšenyo, 'me u e hloekise kapa u e khutlisetse ha ho hlokahala.

* Sheba thermostat 'me u fetole ha ho hlokahala.

Haeba malebela a ka holimo a sa rarolle bothata, ikopanye le setsebi sa setsebi kapa moetsi bakeng sa thuso e eketsehileng. Kamehla latela litaelo tsa moetsi le litataiso tsa polokeho ha u rarolla mathata kapa u lokisa centrifuge ea hau.

Ho fetola lebelo la centrifuge le nako bakeng sa karohano e nepahetseng ho tla itšetleha ka mofuta oa sampole e sebetsoang le ts'ebeliso e reriloeng.

Mehato e meng ea ho e latela ke ena ha u lokisa lebelo la centrifuge le nako bakeng sa karohano e nepahetseng:

1. Etsa qeto ea boima ba sampuli: Boima ba sampuli bo tla khetholla matla a centrifugal a loketseng a hlokahalang bakeng sa karohano e nepahetseng. Ha matla a sampuli a phahame, matla a centrifugal a hlokahalang a phahame.

2. Etsa qeto ea karohano e lakatsehang: Karohano e lakatsehang e tla itšetleha ka mofuta oa sampole e sebetsoang le kopo e reriloeng. Ka mohlala, haeba u arola metsoako ea mali, u ka ’na ua rata ho arola plasma ho lisele tse khubelu tsa mali.

3. Bala matla a centrifugal a hlokahalang: Hang ha u se u entse qeto ea ho teteka ha sampuli le karohano e lakatsehang, bala matla a centrifugal a hlokahalang ka sebali sa matla a centrifugal kapa sheba litaelo tsa moetsi bakeng sa mohlala oa hau oa centrifuge.

4. Beha lebelo le loketseng: Beha lebelo le loketseng ho centrifuge ho latela matla a baloang a centrifugal le lintlha tsa rotor. Lebelo le lokela ho ba holimo ho lekana ho fihlela matla a centrifugal a hlokahalang empa eseng holimo hoo a senyang sampuli kapa thepa.

5. Beha nako e loketseng: Beha nako e loketseng ho centrifuge ho latela mofuta oa mohlala le kopo e reretsoeng. Sena se ka fapana ho latela boholo ba sampuli le karohano e lakatsehang.

6. Lekola ts'ebetso: Lekola ts'ebetso ea centrifuge ho netefatsa hore lisampole li arohane hantle ntle le tšenyo kapa tšilafalo.

7. Fetola ha ho hlokahala: Haeba karohano e sa nepahale, fetola lebelo kapa nako ka mokhoa o nepahetseng 'me u phete mokhoa oo ho fihlela karohano e lakatsehang e finyelloa.

Ka ho latela mehato ena, o ka fetola lebelo la centrifuge le nako bakeng sa karohano e nepahetseng, ho netefatsa liphetho tse nepahetseng le tse tšepahalang bakeng sa ts'ebeliso ea hau e ikhethileng. Ho bohlokoa ho shebana le litaelo tsa moetsi le ho batla tataiso ho tsoa ho litsebi tse nang le boiphihlelo haeba o sa tiisehe ka litlhophiso tse nepahetseng bakeng sa mofuta oa sampole le ts'ebeliso ea hau.

Ho sebetsa le ho hlokomela centrifuge ho hloka koetliso e nepahetseng le ho ela hloko mehato ea polokeho ho thibela likotsi le ho boloka tšebetso ea sesebelisoa.

Mehato e meng ea ho e latela ke ena ha u sebetsa ka mokhoa o sireletsehileng le ho hlokomela centrifuge:

1. Bala litaelo tsa moetsi: Pele u sebelisa centrifuge, bala litaelo tsa moetsi 'me u tloaelane le likarolo tsa thepa le litlhaloso.

2. Apara thepa e loketseng ea ho itšireletsa (PPE): Kamehla u roale PPE e loketseng, joaloka liatlana le tšireletso ea mahlo, ha u sebelisa centrifuge.

3. Laela rotor le li-tubes ka nepo: Latela litaelo tsa moetsi bakeng sa ho kenya rotor le li-tubes, ho etsa bonnete ba hore li leka-lekane ebile li tiisitsoe ka mokhoa o sireletsehileng.

4. Beha lebelo le nako e loketseng: Beha lebelo le nako e loketseng bakeng sa centrifuge ho latela mofuta oa mohlala le kopo e reretsoeng.

5. Qala centrifuge mme u shebe ts'ebetso: Qala centrifuge 'me u shebe ts'ebetso ho netefatsa hore e sebetsa ka nepo le ka mokhoa o sireletsehileng.

6. Emisa centrifuge 'me u tlose lisampole ka mokhoa o sireletsehileng: Hang ha centrifuge e qetile potoloho ea eona, e emise' me u tlose lisampole ka mokhoa o sireletsehileng. Etsa bonnete ba hore o apara PPE e nepahetseng mme o be hlokolosi ha o tshwara disampole.

7. Hloekisa rotor le li-tubes: Kamora ho sebelisoa, hloekisa rotor le li-tubes ho latela litaelo tsa moetsi. Sena se tla thusa ho thibela tšilafalo le ho netefatsa hore thepa e phela nako e telele.

8. Etsa tlhokomelo ea kamehla: Hlahloba le ho boloka centrifuge khafetsa ho netefatsa hore e sebetsa hantle. Sena se ka kenyelletsa ho tlotsa lisebelisoa, ho fetola likarolo tse tsofetseng, le ho lekanya lebelo le litlhophiso tsa nako.

9. Latela mekhoa ea ho lahla: Lahla litšila kapa thepa leha e le efe e kotsi ho latela melaoana e nepahetseng ho thibela tšilafalo le ho sireletsa tikoloho.

Ka ho latela mehato ena, o ka sebetsa le ho boloka centrifuge ea hau ka mokhoa o sireletsehileng, o netefatsa liphetho tse nepahetseng le tse tšepahalang ha o ntse o sireletsa polokeho ea hau le polokeho ea ba bang.

Ho khetha rotor le tube e nepahetseng bakeng sa centrifuge ea hau ho tla itšetleha ka lintlha tse 'maloa, ho kenyelletsa le mofuta oa sampole e sebetsoang, matla a centrifugal a hlokahalang, le molumo oa sampole.

Mehato e meng eo u lokelang ho e latela ke ena ha u khetha rotor le tube e nepahetseng bakeng sa centrifuge ea hau:

1. Etsa qeto ea mofuta oa sampuli e sebelisoang: Mofuta oa sampuli o sebelisoang o tla khetholla mofuta oa tube le rotor e hlokahalang. Ka mohlala, haeba u ntse u sebetsa lisampole tsa likokoana-hloko, mohlomong u tla hloka rotor le tube e ka amohelang li-microcentrifuge tubes. Haeba o ntse o sebetsa ka bongata bo boholo ba metsi, o ka hloka tube e kholoanyane le rotor.

2. Etsa qeto ea matla a hlokahalang a centrifugal: Matla a hlokahalang a centrifugal a tla itšetleha ka mofuta oa mohlala le kopo e reretsoeng. Matla a phahameng a centrifugal a hlokahala bakeng sa lits'ebetso tse itseng, joalo ka ho arola maloanlahla a cellular kapa liprotheine tsa pelleting. Etsa bonnete ba hore rotor le tube eo u e khethang e ka amohela matla a centrifugal a hlokahalang.

3. Etsa qeto ea boholo ba sampuli: Molumo oa sampuli o tla boela o phetha karolo ea ho khetholla mofuta oa rotor le tube e hlokahalang. Haeba o ntse o sebetsa ka bongata bo fokolang ba metsi, li-tubes tsa microcentrifuge li ka lekane. Bakeng sa meqolo e meholo, o ka hloka li-tubes tse kholoanyane le li-rotor.

4. Nahana ka thepa ea rotor le tube: Thepa ea rotor le tube e tla itšetleha ka mofuta oa sampuli e sebelisoang. Ka mohlala, haeba u sebetsana le lisebelisoa tse senyang kapa tse kotsi, ho ka 'na ha hlokahala hore u khethe rotor le tube e entsoeng ka thepa e itseng e ka mamellang lintho tsena.

5. Hlahloba ho lumellana ha rotor le tube le centrifuge ea hau: Etsa bonnete ba hore rotor le tube eo u e khethang e lumellana le mohlala oa hau o khethehileng oa centrifuge. Sheba lintlha tsa moetsi ho netefatsa hore rotor le tube li ka sebelisoa le centrifuge ea hau.

Ka ho latela mehato ena, o ka khetha rotor le tube e nepahetseng bakeng sa centrifuge ea hau mme u netefatse hore lisampole tsa hau li sebetsoa ka nepo le ka nepo.

Ho kenya le ho leka-lekanya lisampole ka har'a centrifuge ke mohato oa bohlokoa ho netefatsa liphetho tse nepahetseng le tse tsitsitseng.

Mehato e meng ke ena ea ho kenya le ho leka-lekanya lisampole ka har'a centrifuge:

1. Lekola boholo ba matla a centrifuge mme o netefatse hore ha o fete moeli ona ha o kenya lisampole.

2. Sebelisa li-tubes tse lekanang le boholo le molumo ho etsa bonnete ba hore ho tšoana ho centrifuge.

3. Etsa bonnete ba hore lisampole li arolelitsoe ka ho lekana ka har'a li-tubes. Sena se ka etsoa ka ho tlanya li-tubes ka bonolo ho lokisa lisampole.

4. Leka-lekanya centrifuge ka ho beha palo e lekanang ea li-tubes mahlakoreng a fapaneng a rotor. Sena se tiisa hore centrifuge e bilika ka ho lekana 'me ha e hlahise ho thothomela ho ka amang ho nepahala ha liphetho.

5. Haeba disampole e le tsa mefuta e fapaneng kapa boima ba 'mele, sebelisa adaptara e loketseng ho leka-lekanya centrifuge. Adapter e lokela ho lumellana le li-tubes le rotor ho netefatsa ho leka-lekana ho nepahetseng.

6. Kamehla latela litaelo tsa moetsi bakeng sa ho kenya le ho leka-lekanya lisampole ka har'a centrifuge. Sena se tla thusa ho qoba tšenyo leha e le efe ho centrifuge le ho netefatsa liphello tse nepahetseng le tse tsitsitseng.

7. Ka mor'a ho kenya lisampole, koala sekoahelo sa centrifuge ka mokhoa o sireletsehileng 'me u hlahlobe hore na se tiisitsoe hantle pele u qala centrifuge.

Ka ho latela mehato ena, o ka etsa bonnete ba hore lisampole li laeloa le ho leka-lekana hantle ka har'a centrifuge, e leng se tla thusa ho netefatsa liphetho tse nepahetseng le tse tsitsitseng.

1, Ho theoha ha vacuum ke ho hloleha ho tloaelehileng. Bakeng sa lebelo le phahameng le li-centrifuges tsa lebelo le phahameng haholo, boholo ba tsona li na le lisebelisoa tsa vacuum, sepheo ke ho boloka kamore ea centrifugal sebakeng se mahareng kapa se phahameng nakong ea ts'ebetso, ho fokotsa ho ferekana hloohong e potolohang le ho etsa hore e tsamaee ka mokhoa o tsitsitseng. Ha boemo ba vacuum bo theoha kapa phallo e se e ntle, hlahloba tsamaiso ea vacuum, hangata oli e tla nkeloa sebaka, 'me boemo ba vacuum bo tla tsosolosoa.

2, centrifuge e nyenyane kapa ea benchtop, hangata e lebala ho kenya kamore ea centrifugal hloohong ea rotor ho ntša, hlooho ea rotor e tla khomarela sefate ka mor'a nako.

3, lebelo le phahameng, ts'ebetso ea ts'ebetso ea centrifuge e phahameng haholo, phanele e na le ts'ebetso ea phoso, o ka sebetsana le ho hloleha ho tsamaisanang le phoso.

Ts'ebetso e nepahetseng, tlhokomelo e utloahalang ke senotlolo sa ho netefatsa ts'ebetso e tloaelehileng ea sesebelisoa le ho lelefatsa bophelo ba tšebeletso ea sesebelisoa. Li-centrifuges tse nang le ts'ebetso e phahameng li lokela ho sebetsoa ka nepo 'me li etse mosebetsi o tloaelehileng oa ho lokisa. Hangata, re lokela ho shebella boemo ba ts'ebetso, ho mamela molumo, ho hlahlojoa kamehla, ho sebetsa ka nako e nepahetseng, o ka fokotsa sekhahla sa ho hlōleha, ho lelefatsa bophelo ba tšebeletso, e le hore centrifuge e bapalle katleho e kholo.

Li-Centrifuge ke lisebelisoa tsa laboratori tse sebelisang matla a centrifugal ho arola likaroloana tse fapaneng tsa likhahla ka har'a mokelikeli kapa khase. Tšebeliso e nepahetseng ea li-centrifuges e bohlokoa ho fumana liphetho tse nepahetseng le tse tšepahalang.

Mona ke litataiso tsa ho sebelisa li-centrifuges ka nepo le ho rarolla mathata a tloaelehileng:

1. Tšebeliso e nepahetseng ea li-tubes tsa centrifuge: Kamehla sebelisa li-tubes tse etselitsoeng ho mamella matla a centrifugal. Lekola lebelo le phahameng la li-tubes pele u li kenya ka har'a rotor ea centrifuge. U se ke ua feta lebelo le phahameng le khothalletsoang kapa matla a boima ba li-tubes.

2. Ho leka-lekanya rotor: Li-rotor tse sa leka-lekaneng li ka etsa hore centrifuge e sisinyehe kapa e etse lerata le leholo, le ka senyang centrifuge kapa sampuli. Kamehla leka-lekanya rotor ka ho beha molumo o tšoanang le boima ba lisampole libakeng tse hanyetsanang tsa tube.

3. Ho qala centrifuge: Sheba sekoahelo kapa sekoahelo sa centrifuge 'me u netefatse hore se notletsoe ka mokhoa o sireletsehileng sebakeng seo. Bulela centrifuge 'me u behe lebelo le nako e nepahetseng ho latela litlhoko tsa mohlala.

4. Ho emisa centrifuge: Emela hore rotor ea centrifuge e eme ka ho feletseng pele e bula sekoahelo. Ho tlosa sekoahelo ha rotor e ntse e bilika ho ka etsa hore lisampole li qhalane kapa rotor e robehe.

5. Ho rarolla mathata a tloaelehileng: Haeba centrifuge e etsa lerata le leholo kapa e thothomela ka mokhoa o feteletseng, emisa centrifuge 'me u hlahlobe ho se leka-lekane ha rotor kapa li-tubes tse senyehileng. Haeba centrifuge e sa bilike kapa e sa bilike butle ho feta kamoo ho neng ho lebelletsoe, hlahloba phepelo ea motlakase, senotlolo sa rotor kapa borashe.

6. Tlhokomelo le ho hloekisa: Kamehla hloekisa rotor ea centrifuge le li-tubes ka sesepa se bonolo le metsi. Sheba rotor le li-tubes bakeng sa matšoao a ho senyeha, 'me u nkele likarolo tse senyehileng sebaka. Lubribetsa enjene ea centrifuge joalo ka ha moetsi a khothalelitse.

Ka kakaretso, tšebeliso e nepahetseng ea li-centrifuges e hloka tlhokomelo e hlokolosi ho khetho ea li-tubes, ho leka-lekanya rotor, ho koala sekoahelo, le lebelo le nako ea nako. Ho rarolla mathata a tloaelehileng ho kenyelletsa ho tseba sesosa sa bothata, joalo ka ho se leka-lekane kapa phepelo ea motlakase, le ho nka mehato e nepahetseng. Tlhokomelo le ho hloekisa khafetsa ho ka thusa ho lelefatsa bophelo ba centrifuge le ho netefatsa liphetho tse nepahetseng le tse tšepahalang.